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G005610

HPLC Analysis of Withania Extract on Ascentis® Express Phenyl-Hexyl

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 10 min; held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 160 bar (2320 psi)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 mg/mL in water:acetonitrile (50:50); sonicate 15 minutes; filter 0.45 μm;
dilute to water:acetonitrile (80:20)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express Phenyl-Hexyl for the efficient separation of withania extract.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Withanoside IV
    Withanoside V
    Withaferin A
    12-Deoxywithastramonolide
    Withanolide A
    Withanone
    Withanolide B

analytical column

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Description
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standard

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