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  • High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.

High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007-04-10)
Jana Maláková, Milan Nobilis, Zbynek Svoboda, Miroslav Lísa, Michal Holcapek, Jaroslav Kvetina, Jirí Klimes, Vladimír Palicka
ABSTRACT

Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation (O-desmethyl-galantamine, N-desmethyl-galantamine, galantamine-N-oxide) and chiral conversion (epigalantamine) are formed in various concentrations from parent compound. For the identification and determination of galantamine and its phase I metabolites in blood plasma and tissues, a new bioanalytical method based on a reversed-phase high-performance liquid chromatography with UV photodiode-array, fluorescence and mass spectrometric detection was developed, validated and applied to pharmacokinetic and biotransformation studies. Sample preparation included a homogenization of the rat tissues (liver, brain, hypophysis) in a phosphate buffer 0.05 mol/L pH 7.4. Plasma samples and tissue homogenates were purified using a mixed-mode solid-phase extraction (Waters Oasis MCX cartridges). Galantamine, its above-mentioned metabolites and the internal standard codeine were separated on a Discovery HS F5 column (Supelco, 150 mmx4.6 mm I.D., 5 microm) at flow rate of 1 mL/min using a linear gradient elution. UV photodiode-array and mass spectrometric detection were employed for the identification of individual galantamine metabolites in various biomatrices, the fluorescence detection (lambdaexcit=280 nm/lambdaemiss=310 nm) was chosen for the quantification of galantamine and its metabolites. The developed method was applicable in liver tissue in the range from 0.50 to 63.47 nmol/g of galantamine, from 0.32 to 41.42 nmol/g of O-desmethyl-galantamine, from 0.54 to 69.40 nmol/g of N-desmethyl-galantamine and from 0.70 to 89.03 nmol/g of epigalantamine. Limit of detection was found to be 0.04 nmol/g for galantamine, 0.19 nmol/g for O-desmethyl-galantamine, and 0.07 nmol/g for N-desmethyl-galantamine and epigalantamine.

MATERIALS
Product Number
Brand
Product Description

Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 3 mm, pkg of 1 ea
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 4 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 1 kit
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm