- Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).
Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).
Chemical communications (Cambridge, England) (2012-02-07)
Musleh M Muthana, Jingyao Qu, Yanhong Li, Lei Zhang, Hai Yu, Li Ding, Hamed Malekan, Xi Chen
PMID22306833
ABSTRACT
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast, Type X, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
Pyrophosphatase, Inorganic from Escherichia coli, recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein
Sigma-Aldrich
Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae), powder, ≥500 units/mg protein (E1%/280)