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Merck
  • Fit-for-purpose biomarker LC-MS/MS qualification for the quantitation of very long chain fatty acids in human cerebrospinal fluid.

Fit-for-purpose biomarker LC-MS/MS qualification for the quantitation of very long chain fatty acids in human cerebrospinal fluid.

Bioanalysis (2020-02-14)
John Williams, Kan Zhu, Eric Crampon, André Iffland
ABSTRACT

Aim: Very long chain fatty acids (VLCFAs) have been identified as biomarkers for several peroxisomal disorders necessitating the need for reliable biomarker assays in particular C20, C22, C24, C26 in cerebrospinal fluid (CSF). Until now no absolute quantitation assay for total VLCFAs in CSF has been successfully developed and qualified for clinical use. Methodology: A quantitative LC-MS/MS assay for total VLCFA in human CSF was developed. Derivatization tag and coupling chemistry were optimized for sensitivity. CSF contamination by blood, non-specific binding of VLCFA to surfaces and exogenous VLCFA contamination was minimized. Discussion/conclusion: This fit for purpose biomarker assay was used to measure baseline healthy human VLCFA levels across multiple subjects to establish an understanding of concentration ranges and feasibility.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-Picolylamine, ≥99%
Supelco
Serotonin, analytical standard
Avanti
18:1-d9 SM, Avanti Research - A Croda Brand
Avanti
26:0-d4 Lyso PC, 1-hexacosanoyl-d4-2-hydroxy-sn-glycero-3-phosphocholine, powder