RAB0197

Human Ferritin ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): FTH, Ferritin H subunit, Ferritin heavy chain
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... FTH1(2495)
• Input: sample type urine; sample type plasma; sample type serum; sample type cell culture supernatant(s)
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type urine; sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 600 pg/mL; standard curve range: 0.81-200 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FTH1(2495)

Description

General description

The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).

Immunogen

Recombinant Human Ferritin

Application

Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.

Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.[1]

Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDS
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids