Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.
NA0200
GenElute™ HP Plasmid Midiprep Kit
sufficient for 25 purifications
Synonym(s):
GenElute™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $421.00 |
About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
$421.00
Check Cart for Availability
usage
sufficient for 25 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
sample volume: 1-5 mL bacterial culture
greener alternative category
, Aligned
storage temp.
15-25°C
General description
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 350 μg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- No phenol/chloroform extraction or alcohol precipitation required
- Contains fewer plastic components, reducing the amount of waste
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| usage sufficient for 25 purifications | usage sufficient for 35 purifications | usage sufficient for 35 purifications | usage sufficient for 10 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| test parameters sample volume: 1-5 mL bacterial culture | test parameters : ~45 min kit run time, sample volume: 5-40 mL bacterial culture | test parameters : <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture | test parameters sample volume: 50 mL bacterial culture |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
signalword
Danger
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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Is plasmid DNA isolated with this kit suitable for use in transfection?
1 answer-
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How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?
1 answer-
The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.
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How long does it take to isolate plasmid using the vacuum format?
1 answer-
A midiprep or maxiprep requires only 30 minutes to complete.
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Which format gives a better yield, vacuum or spin?
1 answer-
There is no difference in plasmid recovery when using either format.
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What is the expected yield of plasmid DNA that you can isolate from these kits?
1 answer-
Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.
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Do the binding columns fit on other manufacturers’ manifolds?
1 answer-
Yes. The columns fit on all standard vacuum manifolds.
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