B5161

Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt

≥90% (TLC), powder

• Synonym(s): Sulfosuccinimidyl biotin
• Empirical Formula (Hill Notation): C₁₄H₁₈N₃NaO₈S₂
• CAS Number: 119616-38-5
• Molecular Weight: 443.43
• MDL number: MFCD00078535
• UNSPSC Code: 12352203
• PubChem Substance ID: 24891813
• NACRES: NA.46

Properties

• Assay: ≥90% (TLC)
• form: powder
• solubility: H₂O: 10 mg/mL; DMF: 50 mg/mL
• storage temp.: −20°C
• SMILES string: [Na].OS(=O)(=O)C1CC(=O)N(OC(=O)CCCCC2SCC3NC(=O)NC23)C1=O
• InChI: 1S/C14H19N3O8S2.Na.H/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);;
• InChI key: NKHCQKKDLWGBRK-UHFFFAOYSA-N

Description

General description

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Application

Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H302 + H312 + H332,H315,H319,H335,H361
• Precautionary Statements: P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves