B3111

ANTI-FLAG^® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

• Synonym(s): Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: M2, monoclonal
• Species reactivity: -
• Application: —
• Citations: 19

Properties

• biological source: mouse
• antibody form: purified immunoglobulin (purified IgG1 subclass)
• clone: M2, monoclonal
• shelf life: 4 yr
• purified by: using Protein A
• storage temp.: −20°C

Description

General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG^® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG^® application portal.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable