2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Synonym(s): Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• CAS Number: 9001-15-4
• EC Number: 2.7.3.2 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.21
• MDL number: MFCD00130876
• Specific activity: ≥300 U/mg, 25 °C, 750 U/mg, 37 °C
• Biological source: rabbit skeletal muscle

Properties

• biological source: rabbit skeletal muscle
• Quality Segment: 200
• form: lyophilized
• specific activity: ≥300 U/mg, 25 °C, 750 U/mg, 37 °C
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• solubility: 0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO₃, pH 9.0: soluble
• foreign activity: ATPase, hexokinase, myokinase <0.001%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Preparation Note

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Other Notes

Bessman, S.P. 1987. Anal. Biochem.161, 519.

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable