04-902

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

culture supernatant, clone DW2, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Clone: DW2, monoclonal
• Species reactivity: vertebrates (common species)
• Application: WB
• Citations: 17

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: DW2, monoclonal
• species reactivity: vertebrates (common species)
• technique(s): western blot: suitable
• isotype: IgG
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Various

Immunogen

KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

Application

Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

Biochem/physiol Actions

Reactivity with other species has not been confirmed.

Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

Physical form

Cultured supernatant in 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HA-tagged WNT-1 protein.

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Other Notes

Replaces: 05-902

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable