Y1250

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

• Synonym(s): ynb with amino acids
• UNSPSC Code: 41106212
• NACRES: NA.85

Properties

• grade: for molecular biology
• sterility: non-sterile
• form: powder
• technique(s): microbiological culture: suitable
• pH: 5.3-5.5(0.67% solution)
• application(s): microbiology
• storage temp.: room temp
• suitability: nonselective for Candida spp.; nonselective for Pichia spp.; nonselective for Saccharomyces spp.; nonselective for Zygosaccharomyces spp.

Description

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Application

Yeast Nitrogen Base With Amino Acids has been used:

• in the ZYP-31 media preparation for E.coli
• in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source
• to culture Candida utilis for the production of ¹³C-labeled yeast extract as internal standard
• in the preparation of imaging medium

Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves