CC165

ECMatrix-332 E8 Laminin Substrate

• Synonym(s): ECMatrix-332 E8 Laminin Substrate, iMatrix-111
• UNSPSC Code: 41106514
• NACRES: NA.75

Properties

• biological source: human
• Quality Level: 100
• packaging: vial of 1050 μg
• manufacturer/tradename: Millipore
• Beta value: 1) 6 X 175 µg ECMatrix-332 E8 Laminin Substrate (0.5 mg/mL in PBS). Expressed in CHO-S cells.Expressed in CHO-S cells.
• shipped in: 2-8°C
• storage temp.: 2-8°C

Description

General description

Laminin 332 (ECMatrix-332) is a large multidomain molecule involved in cell adhesion and matrix assembly. Laminin 332 supports cells in the epithelial basement membranes, lining the surfaces of the body such as the skin, hair follicles, oral cavity, gastrointestinal and urinary tracts, lungs, and different glands. The association of cells with laminin 332 occurs through α3β1 integrin in focal adhesions and α6β4 in stable anchoring contacts, which contain an assembly of hemidesmosome proteins.Proteolytic processing of the γ2 and α3 chains of laminin 332 has been linked to cell migration and invasion. Members of the astacin family process the γ2chain, and various enzymes, including astacin enzymes, matrix metalloproteinase 2 (MMP2), MT1-MMP and plasmin process the α3 chain.It is an important component of the "skin basement membrane" that exists between the epithelium and dermis of the skin, and is also expressed in human retinal pigment epithelium (RPE) and hair follicle cells.References1. S Shibata, et al. Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium. Stem Cell Reports. 2020 Apr 14;14(4):663-676. 2. S Shibata, et al. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell reports. 2018 Nov 6;25(6):1668-1679.e5.

Application

• Purity (SDS-Page): <gt/> 95%
• Endotoxin Test: < 750 EU/mg
• Mycoplasma Test: Negative
• Sterility Test: Negative
• Integrin Binding Assay (kDa): < 10 nM

Features and Benefits

Xeno-free laminin-332 ECM coating for many epithelial cell types including skin keratinocytes, hair, RPE, oral, urinary, gastrointestinal and lung cells.

Storage and Stability

ECMatrix-332 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable