Skip to Content
Merck

SYK kinase mediates brown fat differentiation and activation.

Nature communications (2017-12-14)
Marko Knoll, Sally Winther, Anirudh Natarajan, Huan Yang, Mengxi Jiang, Prathapan Thiru, Aliakbar Shahsafaei, Tony E Chavarria, Dudley W Lamming, Lei Sun, Jacob B Hansen, Harvey F Lodish
ABSTRACT

Brown adipose tissue (BAT) metabolism influences glucose homeostasis and metabolic health in mice and humans. Sympathetic stimulation of β-adrenergic receptors in response to cold induces proliferation, differentiation, and UCP1 expression in pre-adipocytes and mature brown adipocytes. Here we show that spleen tyrosine kinase (SYK) is upregulated during brown adipocyte differentiation and activated by β-adrenergic stimulation. Deletion or inhibition of SYK, a kinase known for its essential roles in the immune system, blocks brown and white pre-adipocyte proliferation and differentiation in vitro, and results in diminished expression of Ucp1 and other genes regulating brown adipocyte function in response to β-adrenergic stimulation. Adipocyte-specific SYK deletion in mice reduces BAT mass and BAT that developed consisted of SYK-expressing brown adipocytes that had escaped homozygous Syk deletion. SYK inhibition in vivo represses β-agonist-induced thermogenesis and oxygen consumption. These results establish SYK as an essential mediator of brown fat formation and function.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Rosiglitazone, ≥98% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
TWEEN® 20, viscous liquid
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Insulin from bovine pancreas, γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, pH 7, ≥98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Triciribine hydrate, ≥97% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
SKU
Pack Size
Availability
Price
Quantity
SAFC
Dulbecco′s Modified Eagle′s Medium/High Modified, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Triton X-100, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
ECO TWEEN® 20, viscous liquid
SKU
Pack Size
Availability
Price
Quantity