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  • Diacylglycerols mimic phorbol diester induction of leukemic cell differentiation.

Diacylglycerols mimic phorbol diester induction of leukemic cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America (1985-02-01)
J G Ebeling, G R Vandenbark, L J Kuhn, B R Ganong, R M Bell, J E Niedel
ABSTRACT

Activation of cellular protein kinase C appears to be involved in the mechanism by which phorbol diesters induce differentiation of human myeloid leukemia cells (HL-60). Protein kinase C is thought to be physiologically activated by diacylglycerol derived from receptor-mediated phosphatidylinositol hydrolysis. sn-1,2-diacylglycerols with short saturated acyl side chains (C4-C10) were synthesized and found to be potent activators of protein kinase C partially purified from HL-60 cells. These diacylglycerols were also competitive inhibitors of [3H]phorbol dibutyrate binding to the soluble phorbol diester receptor. The most potent diacylglycerol, sn-1,2-dioctanoylglycerol, displaced greater than 90% of [3H]phorbol dibutyrate from the phorbol diester receptor of intact HL-60 cells. Because of probable cellular metabolism of sn-1,2-dioctanoylglycerol, hourly doses were required to maintain persistent occupancy of the phorbol diester binding site. Treatment of HL-60 cells with either phorbol 12-myristate 13-acetate or sn-1,2-dioctanoylglycerol produced identical phosphoprotein changes. Finally, sn-1,2-dioctanoylglycerol induced differentiation of the HL-60 cells into cells with morphologic characteristics of macrophages. Substitution of the hydroxyl group at position 3 with a hydrogen, chloro, or sulfhydryl moiety inactivated sn-1,2-dioctanoylglycerol. These data strengthen the hypothesis that protein kinase C activation plays a role in macrophage differentiation.

MATERIALS
Product Number
Brand
Product Description

Avanti
18:1 DG, Avanti Research - A Croda Brand
Avanti
18:0-20:4 DG, 1-stearoyl-2-arachidonoyl-sn-glycerol, chloroform
Avanti
18:0-18:2 DG, 1-stearoyl-2-linoleoyl-sn-glycerol, chloroform
Avanti
18:1 DG, 1-2-dioleoyl-sn-glycerol, chloroform
Avanti
08:0 DG, 1,2-dioctanoyl-sn-glycerol, chloroform solution
Avanti
16:0-18:1 DG, 1-palmitoyl-2-oleoyl-sn-glycerol, chloroform
Avanti
08:0 DG, 1,2-dioctanoyl-sn-glycerol, neat oil
Avanti
16:0-18:1 DG, 1-palmitoyl-2-oleoyl-sn-glycerol, neat oil
Avanti
10:0 DG, 1,2-didecanoyl-sn-glycerol, powder
Avanti
16:0 DG, 1,2-dipalmitoyl-sn-glycerol, powder
Avanti
16:0 DG, 1,2-dipalmitoyl-sn-glycerol, chloroform
Avanti
14:0 DG, 1,2-dimyristoyl-sn-glycerol, powder
Avanti
18:0 DG, 1,2-dioctadecanoyl-sn-glycerol, powder
Avanti
18:0-22:6 DG, 1-stearoyl-2-docosahexaenoyl-sn-glycerol, chloroform
Avanti
12:0 DG, Avanti Research - A Croda Brand
Avanti
18:1-2:0 DG, 1-oleoyl-2-acetyl-sn-glycerol, chloroform
Avanti
18:1-2:0 DG, 1-oleoyl-2-acetyl-sn-glycerol, neat oil