- Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.
Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.
Nature methods (2020-07-01)
Sebastian Strauss, Ralf Jungmann
PMID32601424
ABSTRACT
DNA-PAINT's imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.
MATERIALS
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Protocatechuate 3,4-Dioxygenase from Pseudomonas sp., lyophilized powder, ≥3 units/mg solid
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CRISPR/Cas9 Products and Services, Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.