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  • Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF.

Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF.

Cell (1997-03-21)
M van de Wetering, R Cavallo, D Dooijes, M van Beest, J van Es, J Loureiro, A Ypma, D Hursh, T Jones, A Bejsovec, M Peifer, M Mortin, H Clevers
PMID9118222
ABSTRACT

The vertebrate transcription factors TCF (T cell factor) and LEF (lymphocyte enhancer binding factor) interact with beta-catenin and are hypothesized to mediate Wingless/Wnt signaling. We have cloned a maternally expressed Drosophila TCF family member, dTCF. dTCF binds a canonical TCF DNA motif and interacts with the beta-catenin homolog Armadillo. Previous studies have identified two regions in Armadillo required for Wingless signaling. One of these interacts with dTCF, while the other constitutes a transactivation domain. Mutations in dTCF and expression of a dominant-negative dTCF transgene cause a segment polarity phenotype and affect expression of the Wingless target genes engrailed and Ultrabithorax. Epistasis analysis positions dTCF downstream of armadillo. The Armadillo-dTCF complex mediates Wingless signaling as a bipartite transcription factor.

MATERIALS
Product Number
Brand
Product Description
21-170
Sigma-Aldrich
TOPflash (TCF Reporter Plasmid), Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.
17-285
Sigma-Aldrich
TCF Reporter Plasmid Kit, Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.
21-169
Sigma-Aldrich
FOPflash (mutant TCF binding sites), Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negative control to TOPflash.