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  • Exogenous sn-1,2-diacylglycerols containing saturated fatty acids function as bioregulators of protein kinase C in human platelets.

Exogenous sn-1,2-diacylglycerols containing saturated fatty acids function as bioregulators of protein kinase C in human platelets.

The Journal of biological chemistry (1985-02-10)
E G Lapetina, B Reep, B R Ganong, R M Bell
ABSTRACT

The ability of exogenous sn-1,2-diacylglycerols and analogs to function as bioregulators of protein kinase C in human platelets was investigated. The activation of protein kinase C in platelets is indicated by specific phosphorylation of a 40,000-dalton protein. Dihexanoylglycerol, dioctanoylglycerol (diC8), didecanoylglycerol, and sn-1-oleoyl-2-acetylglycerol were active in stimulating 40,000-dalton protein phosphorylation. Only a trace of phosphorylation was elicited by dibutyrylglycerol. Phosphorylation was not induced by analogs of diC8 in which an -H, -SH, or -Cl group replaced the free -OH, nor by monoacylglycerols or long chain diacylglycerols. Maximum phosphorylation was induced by dihexanoylglycerol, diC8, and didecanoylglycerol at concentrations from 5 to 20 microM and between 5 and 30 S after exposure of platelets to these diacylglycerols. Under conditions of maximal phosphorylation of the 40,000-dalton protein, these diacylglycerols did not induce phosphatidylinositol turnover, or platelet aggregation, or stimulate release of ATP or serotonin. A small degree of aggregation was evident with platelets isolated in the absence of prostacyclin, and release of serotonin was observed when 1 mM Ca2+ or submaximal concentrations of ionophore A23187 were included. These results are consistent with a model in which platelet activation requires the simultaneous formation of two intracellular signals, diacylglycerols and Ca2+. These diacylglycerols and diacylglycerol analogs provide useful tools to investigate the function of diacylglycerols as bioregulators in intact cells.

MATERIALS
Product Number
Brand
Product Description

Avanti
18:1 DG, Avanti Research - A Croda Brand
Avanti
18:0-20:4 DG, 1-stearoyl-2-arachidonoyl-sn-glycerol, chloroform
Avanti
18:0-18:2 DG, 1-stearoyl-2-linoleoyl-sn-glycerol, chloroform
Avanti
18:1 DG, 1-2-dioleoyl-sn-glycerol, chloroform
Avanti
08:0 DG, 1,2-dioctanoyl-sn-glycerol, chloroform solution
Avanti
16:0-18:1 DG, 1-palmitoyl-2-oleoyl-sn-glycerol, chloroform
Avanti
08:0 DG, 1,2-dioctanoyl-sn-glycerol, neat oil
Avanti
16:0-18:1 DG, 1-palmitoyl-2-oleoyl-sn-glycerol, neat oil
Avanti
10:0 DG, 1,2-didecanoyl-sn-glycerol, powder
Avanti
16:0 DG, 1,2-dipalmitoyl-sn-glycerol, powder
Avanti
16:0 DG, 1,2-dipalmitoyl-sn-glycerol, chloroform
Avanti
14:0 DG, 1,2-dimyristoyl-sn-glycerol, powder
Avanti
18:0 DG, 1,2-dioctadecanoyl-sn-glycerol, powder
Avanti
18:0-22:6 DG, 1-stearoyl-2-docosahexaenoyl-sn-glycerol, chloroform
Avanti
12:0 DG, Avanti Research - A Croda Brand
Avanti
18:1-2:0 DG, 1-oleoyl-2-acetyl-sn-glycerol, chloroform
Avanti
18:1-2:0 DG, 1-oleoyl-2-acetyl-sn-glycerol, neat oil