SAB4503329
Anti-DARPP-32 antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 22 kDa
species reactivity
mouse, rat, human
concentration
~1 mg/mL
technique(s)
ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PPP1R1B(84152)
General description
Anti-DARPP-32 Antibody detects endogenous levels of total DARPP-32 protein.
PPP1R1B (protein phosphatase 1 regulatory inhibitor subunit 1B)/DARPP32 (dopamine- and cAMP-regulated phosphoprotein of molecular weight 32 kDa) encodes two transcripts which are further translated into two DARPP-32 protein isoforms, full length (fl)-DARPP-32 and truncated (t)-DARPP. It is located on human chromosome 17q12.
The DARPP-32 (dopamine and cAMP-regulated neuronal phosphoprotein 2) gene is mapped to human chromosome 17q12. The gene is expressed predominantly in spiny neurons of the neostriatum and specifically localized in caudate, putamen, nucleus accumbens, cerebral and cerebellar cortex. The gene encodes two isoforms, full length (fl)-DARPP-32 and truncated (t)-DARPP. The lack of the first 36 residues at the N-terminal in t- DARPP marks the difference between the two isoforms. Anti-DARPP-32 Antibody detects endogenous levels of total DARPP-32 protein.
Immunogen
The antiserum was produced against synthesized peptide derived from human DARPP-32.
Immunogen Range: 41-90
Immunogen Range: 41-90
Biochem/physiol Actions
DARPP-32 (dopamine and cAMP-regulated neuronal phosphoprotein 2) is a dopaminergic gene that influences dopamine receptor binding potential in the corpus striatum. The gene also regulates the availability of dopamine neurotransmitter, which is important for cognitive capacities. Therefore, variation in this gene affects category learning performance. (t)-DARPP takes part in the process of tumorigenesis by stimulating chemokine receptor 4 and also promoting cell proliferation. Elevated expression of the DARPP-32 gene in dorsolateral prefrontal neocortex and hippocampus is correlated with the development of schizophrenia and cognitive disorders. The encoded protein is a phosphorylation-dependent inhibitor. DARPP-32 is associated with the pathogenesis of gastric cancer and breast cancer with its anti-apoptotic property, and is found to promote angiogenesis.
PPP1R1B (protein phosphatase 1 regulatory inhibitor subunit 1B)/DARPP32 (dopamine- and cAMP-regulated phosphoprotein of molecular weight 32 kDa) is considered as an inhibitor of phosphate-1 (PP-1) protein. It is associated with working memory and emotion processing.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Darpp-32: a novel antiapoptotic gene in upper gastrointestinal carcinomas.
Belkhiri A, et al.
Cancer Research, 65(15), 6583-6592 (2005)
Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis.
Chen Z, et al.
Gut (2015)
Jay V Deng et al.
Neuroscience, 369, 212-221 (2017-11-21)
Many neuropsychiatric disorders are associated with abnormal decision making involving risk of punishment, but the underlying molecular basis remains poorly understood. Methyl CpG-binding protein 2 (MeCP2) is an epigenetic factor that regulates transcription by directly binding to methylated DNA. Here
Variation of the gene coding for DARPP-32 (PPP1R1B) and brain connectivity during associative emotional learning.
Curcic-Blake B, et al.
Neuroimage, 59(2), 1540-1550 (2012)
Integrated gene copy number and expression microarray analysis of gastric cancer highlights potential target genes.
Myllykangas S, et al.
International Journal of Cancer. Journal International Du Cancer, 123(4), 817-825 (2008)
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