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RAB0288

Sigma-Aldrich

Rat IL-2 ELISA Kit

for serum, plasma, cell culture supernatant

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.12-30 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Il2(116562)

General description

The Rat IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in serum, plasma, cell culture supernatants and urine.
IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells.

Immunogen

Recombinant Rat IL-2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat IL-2 ELISA Kit has been used to measure the presence of IL-2 in in rat colonic tissue.

Biochem/physiol Actions

IL-2 (interleukin-2) expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 cells and endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Decreased c-rel activation contributes to aberrant interleukin-2 expression in CD4+ T cells of aged rats.
Gong Z, et al.
Molecular Immunology, 61(1), 1-6 (2014)
Acute exhaustive exercise regulates IL-2, IL-4 and MyoD in skeletal muscle but not adipose tissue in rats.
Neto J, et al.
Lipids in Health and Disease, 10(1), 97-97 (2011)
Novel 1, 4-benzothazines obliterate COX-2 mediated JAK-2/STAT-3 signals with potential regulation of oxidative and metabolic stress during colorectal cancer.
Rai A, et al.
Pharmacological Research, 132, 188-203 (2018)
Pranesh Kumar et al.
Regulatory toxicology and pharmacology : RTP, 109, 104489-104489 (2019-10-13)
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwideand haslimited treatment options. In view of this, zafirlukast (ZAF) was administered orally to DEN-induced HCC rats to evaluate its antineoplastic properties. ELISA, qRT-PCR and Western blot were used to
Dynamics of the IL-2 cytokine network and T-cell proliferation. (2010)
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