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CHP1

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Imprint® Chromatin Immunoprecipitation Kit

Complete ChIP reaction in 6 hours in flexible strip well format

Synonym(s):

Chromatin Immunoprecipitation

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.54

storage temp.

−20°C

Related Categories

General description

Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.

For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.

Application

The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
  • Sensitive—As few as 10,000 cells required for each ChIP sample
  • Convenient—Fewest steps of any available ChIP protocol
  • Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
  • Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology

Legal Information

GenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS
  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mLSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

Certificates of Analysis (COA)

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Thomas Palm et al.
Nucleic acids research, 41(6), 3699-3712 (2013-02-12)
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Poulomi Banerjee et al.
Journal of molecular medicine (Berlin, Germany), 94(12), 1397-1409 (2016-08-19)
Epithelial-mesenchymal transition (EMT) is one of the key biological phenomena behind cancer and metastasis. Clinical studies suggest that patients undergoing metformin therapy are less predisposed to cancer but the underlying mechanism is far from clear. Given that metformin also acts
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
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Cancer research, 72(8), 1929-1934 (2012-02-18)
The AC133 epitope has been used as a marker for both normal and cancer stem cells from multiple tissue lineages. To identify transcription factors that regulate CD133 expression, we conducted parallel large-scale RNA interference screens in Caco-2 cancer cells that
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Protocols

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

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