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MAB10234-I

Sigma-Aldrich

Anti-Hepatitis B Virus Surface Antigen Antibody, clone H21F8-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.74

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

H21F8-1, monoclonal

mol wt

calculated mol wt 43.72 kDa
observed mol wt ~43.72 kDa

species reactivity

virus

packaging

antibody small pack of 100 μg

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgM

epitope sequence

Unknown

UniProt accession no.

shipped in

2-8°C

target post-translational modification

unmodified

Gene Information

vaccinia virus ... S(944569)

General description

Large envelope protein (UniProt: A7XR30; also known as L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen) is encoded by the S (also known as Large S, P, preS1, preS2) gene (Gene ID: 944569) in Hepatitis B virus (HBV). HBV is a small enveloped virus of the Hepadnaviridae family with a partially double-stranded DNA genome approximately 3.2 kb. Hepatitis B surface antigen is a distinctive serological marker of acute or chronic hepatitis B infection and is the first antigen to appear following infection with hepatitis B virus. HBV contains three viral envelope/surface proteins - large surface antigen, middle surface antigen, and small surface antigen. They are expressed from a single open reading frame. The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and is not N-glycosylated. It is shown to exist in two topological conformations, one which is termed ′external′ or Le-HBsAg and the other ′internal′ or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. (Ref.: Wu, CC., et al. (2018). World J. Gastroenterol. 24(31);: 3488 3499).

Specificity

Clone H21F8-1 is a mouse monoclonal antibody that detects large envelope protein of Hepatitis B virus.

Immunogen

Hepatitis B virus surface antigen (HBsAg).

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Hepatitis B Virus Surface Antigen in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.
Anti-Hepatitis B Virus Surface Antigen, clone H21F8-1, Cat. No. MAB10234-I, is a mouse monoclonal antibody that detects Large envelope protein of Hepatitis B virus and is tested for use in Immunohistochemistry (Paraffin).

Physical form

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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