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T1283

Sigma-Aldrich

holo-Transferrin bovine

Iron-saturated, BioReagent, suitable for cell culture

Synonym(s):

Siderophilin, Siderophilin, iron-saturated

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About This Item

CAS Number:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00131337
UNSPSC Code:
12352202
NACRES:
NA.75

product line

BioReagent

Assay

97-100% (agarose gel electrophoresis)

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

Q29443

shipped in

ambient

storage temp.

2-8°C

Gene Information

bovine ... TF(280705)

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Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Bovine holo-transferrin can be used with a wide range of cells types. Bovine holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Analysis Note

Purity by agarose gel electrophoresis.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Han Hu et al.
Animal science journal = Nihon chikusan Gakkaiho, 87(1), 84-91 (2015-10-16)
In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42°C for 0.5, 1, 3, 5, 8 or 12 h, and 38°C as control. The transcription of the genes (HSP27, HSP70 and
Samuel G Rodriques et al.
Nature biotechnology, 39(3), 320-325 (2020-10-21)
Current approaches to single-cell RNA sequencing (RNA-seq) provide only limited information about the dynamics of gene expression. Here we present RNA timestamps, a method for inferring the age of individual RNAs in RNA-seq data by exploiting RNA editing. To introduce
William Schachterle et al.
Nature communications, 8, 13963-13963 (2017-01-17)
Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little
Anastassia K Pogoutse et al.
Journal of bacteriology, 202(14) (2020-05-06)
The bacterial bipartite transferrin receptor is an iron acquisition system that several important human and animal pathogens require for survival. It consists of the TonB-dependent transporter transferrin binding protein A (TbpA) and the surface lipoprotein transferrin binding protein B (TbpB).
Wenting Dai et al.
The British journal of nutrition, 123(5), 489-498 (2019-11-13)
Despite the well-characterised mechanisms of amino acids (AA) regulation of milk protein synthesis in mammary glands (MG), the underlying specific AA regulatory machinery in bovine MG remains further elucidated. As methionine (Met) is one of the most important essential and
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