- Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.
Nature methods (2014-03-19)
Johan Hattne, Nathaniel Echols, Rosalie Tran, Jan Kern, Richard J Gildea, Aaron S Brewster, Roberto Alonso-Mori, Carina Glöckner, Julia Hellmich, Hartawan Laksmono, Raymond G Sierra, Benedikt Lassalle-Kaiser, Alyssa Lampe, Guangye Han, Sheraz Gul, Dörte DiFiore, Despina Milathianaki, Alan R Fry, Alan Miahnahri, William E White, Donald W Schafer, M Marvin Seibert, Jason E Koglin, Dimosthenis Sokaras, Tsu-Chien Weng, Jonas Sellberg, Matthew J Latimer, Pieter Glatzel, Petrus H Zwart, Ralf W Grosse-Kunstleve, Michael J Bogan, Marc Messerschmidt, Garth J Williams, Sébastien Boutet, Johannes Messinger, Athina Zouni, Junko Yano, Uwe Bergmann, Vittal K Yachandra, Paul D Adams, Nicholas K Sauter
PMID24633409
ABSTRACT
X-ray free-electron laser (XFEL) sources enable the use of crystallography to solve three-dimensional macromolecular structures under native conditions and without radiation damage. Results to date, however, have been limited by the challenge of deriving accurate Bragg intensities from a heterogeneous population of microcrystals, while at the same time modeling the X-ray spectrum and detector geometry. Here we present a computational approach designed to extract meaningful high-resolution signals from fewer diffraction measurements.
MATERIALS
Product Number
Brand
Product Description
Zinc, foil, 100x100mm, thickness 6mm, as rolled, 99.99+%
Zinc, foil, 25x25mm, thickness 3mm, as rolled, 99.99+%
Zinc, foil, 25x25mm, thickness 6mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 0.0025mm, 99.9%
Zinc, foil, 10mm disks, thickness 0.125mm, as rolled, 99.95+%
Zinc, foil, 50x50mm, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.25mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 1.0mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.05mm, as rolled, 99.99+%
Zinc, foil, 15mm disks, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 0.5m coil, thickness 0.05mm, as rolled, 99.95+%
Zinc, foil, 1m coil, thickness 0.125mm, as rolled, 99.95+%
Zinc, foil, 25mm disks, thickness 0.025mm, as rolled, 99.99+%
Zinc, foil, 25mm disks, thickness 0.20mm, as rolled, 99.95+%
Zinc, foil, 25mm disks, thickness 0.05mm, as rolled, 99.99+%
Zinc, foil, 1m coil, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 4mm disks, thickness 0.5mm, as rolled, 99.99+%
Zinc, foil, 50mm disks, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 5m coil, thickness 0.05mm, as rolled, 99.95+%
Zinc, foil, 4mm disks, thickness 1.0mm, as rolled, 99.95+%
Zinc, foil, 25mm disks, thickness 0.35mm, as rolled, 99.95+%
Zinc, foil, 4mm disks, thickness 1.0mm, as rolled, 99.99+%
Zinc, foil, 50mm disks, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 4mm disks, thickness 0.175mm, as rolled, 99.95+%
Zinc, foil, 2m coil, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 5m coil, thickness 0.125mm, as rolled, 99.95+%
Zinc, foil, 25mm disks, thickness 0.5mm, as rolled, 99.99+%
Zinc, foil, 2m coil, thickness 0.20mm, as rolled, 99.95+%
Zinc, foil, 8mm disks, thickness 0.15mm, as rolled, 99.95+%