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  • [Long-term preservation of DNA in aqueous solutions in the presence of the chemical analogues of microbial autoregulators].

[Long-term preservation of DNA in aqueous solutions in the presence of the chemical analogues of microbial autoregulators].

Mikrobiologiia (2006-11-10)
O K Davydova, D G Deriabin, G I El'-Registan
ABSTRACT

The fact of long-term preservation of the physicochemical properties of DNA molecules in aqueous solutions in complexes with methylresorcinol, hexylresorcinol, and tyrosol, the chemical analogues of microbial autoregulators (d1 factors) from the group of alkylhydroxybenzenes (AOB), was established. Compared to the control variants of storage of aqueous DNA solutions, the AOB influence consisted in the sum of correlating effects: the prevention of DNA degradation (according to spectrophotometric parameters) and the preservation of its viscous characteristics and electrophoretic mobility. The initial DNA properties were preserved to the greatest degree in the presence of hexylresorcinol, the compound with the longest alkyl radical. Possible mechanisms of the protective action of alkylhydroxybenzenes in relation to DNA are discussed, namely, the prevention of its hydrolysis due to isolation from the aqueous environment and maintaining DNA stability in the dormant forms of microorganisms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Methylresorcinol, 98%
Sigma-Aldrich
4-Hexylresorcinol, 98%