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794-0036

HPLC Analysis of pBr322 DNA-Hae III Digest on TSKgel® DEAE-NPR

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: TSKgel DEAE-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (with 5 mm DEAE-NPR guard column) (813075)
mobile phase: [A] 1 M sodium chloride/25 mm Tris-HCl, pH 9.0; [B] 25 mM Tris-HCl, pH 9.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 260 nm
injection: 20 μL of 50 μg/mL Hae III digest of pBR322 DNA

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Description
    pBR322 Hae III Digest, lyophilized powder

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Rapid separation, quantitation, and purification of products of polymerase chain reaction by liquid chromatography
Elena D. Katz, Lawrence A. Haff, Roy Eksteen
Journal of Chromatography A, 512, 433-444 (1990)

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