MABS1252

Anti-Polycystin-1 Antibody, clone E4 (5F4D2)

clone E4 (5F4D2), from mouse

• Synonym(s): Polycystin-1, Autosomal dominant polycystic kidney disease 1 protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: E4 (5F4D2), monoclonal
• species reactivity: human, mouse, rat
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001009944.2
• UniProt accession no.: P98161
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PKD1(5310)

Description

General description

Polycystin-1 (UniProt P98161; also known as Autosomal dominant polycystic kidney disease 1 protein) is encoded by the PKD1 gene (Gene ID 5310) in human. The primary cilium is a microtubule-based mechano- and chemo-sensory organelle that coordinates an array of cellular pathways during development and in tissue homeostasis, including Hedgehog (Hh), PDGFRα and Wnt signaling. Defective ciliary function causes a variety of ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). Polycystin-1 (PC1) and polycystin-2 (PC2) are large transmembrane proteins co-localized to primary cilium, where they play an important role in calcium-based signalling. PC1 is an atypical adhesion G-protein-coupled receptor (aGPCR) with 11-transmemrbane domains. PC1 is cis-autoproteolytically cleaved at a juxtamembrane GPCR cleavage site (GPS; a.a. 3012-3061) in a developmental stage-dependent manner, where PC1 is largely uncleaved in early embryonic kidneys but becomes extensively cleaved after birth. GPS cleavage results in a heterodimeric PC1 form, in which the N-terminal fragment (NTF) remains non-covalently associated with the transmembrane C-terminal fragment (CTF). Transgenic mice expressing non-cleavable PC1 develop cystic kidney disease during the postnatal period.

Specificity

Clone E4 (also known as clone 5F4D2) targets polycystin-1 extracellular C-type lectin domain and immunostained wild-type, but not Pkd1-knockout murine cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).

Immunogen

Epitope: C-type lectin domain.

FLAG-tagged recombinant human polycystin-1 C-type lectin domain.

Application

Anti-Polycystin-1 Antibody, clone E4 (5F4D2) is an antibody against Polycystin-1 for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry.

Immunohistochemistry Analysis: An 1:50 dilution of this antibody from a representative lot detected polycystin-1 immunoreactivity in rat and mouse kidney tissue sections.
Immunoprecipitation Analysis: A representative lot immunoprecipitated polycystin-1 from mouse lung tissue lysates (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected exogenously expressed polycystin-1 in lysates from HEK cells transfected with human or mouse Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected the polycystin-1 (PC1) GPS domain cis-autocleaved N-terminal fragment (NTF) in lysates from HEK cells transfected with full-length Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
Immunocytochemistry Analysis: A representative lot and an anti-Arl13b antibody co-stained the primary cilium of 4% formaldehyde-fixed, 0.1% Triton X-100-permeabilized murine embryonic fibrolasts (mMEF) and collecting duct (CD)-derived cells by dual fluorescence immunocytochemistry. No polycystin-1 immunoreactivity was detected in Pkd1-knockout mMEFs (Kim, H., et al. (2014). Nat. Commun. 5:5482).

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: An 1:250 dilution of this antibody lot detected polycystin-1 immunoreactivity in human kidney tissue.

Target description

Calculated molecular weights of full-length/GPS domain autocleaved N-terminal fragment: 460.3/325.0 kDa (isoform 1), 459.1/324.0 kDa (isoform 2), 460.2/325.0 kDa (isoform 3).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable