MABN2709
Anti-LRAT Antibody, clone 49569
Synonym(s):
lecithin retinol acyl transferase, retinyl ester synthase
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
49569, monoclonal
mol wt
calculated mol wt 25.820 kDa
observed mol wt ~24 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
Cytoplasmic domain
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
mouse ... Lrat(79235)
Specificity
Clone 49569 is a mouse monoclonal antibody that detects LRAT. It targets an epitope within 91 amino acids from the cytoplasmic domain of the protein.
Immunogen
His-tagged recombinant mouse LRAT fragment corresponding to 91 amino acids from the cytoplasmic domain of mouse LRAT.
Application
Quality Control Testing
Evaluated by Western Blotting in mouse retina lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.
Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in mouse retina lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.
Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).
Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Lecithin retinol acyltransferase (UniProt: Q9JI60) (also known as EC:2.3.1.135) is encoded by the Lrat gene (Gene ID:79235) in mouse. Lecithin-retinol acyltransferase (LRAT) is an essential enzyme involved in the metabolism of retinoids, particularly vitamin A. LRAT contains a conserved domain crucial for its enzymatic activity. The primary function of LRAT is to catalyze the esterification of retinol to produce retinyl esters, a process vital for the storage and transport of vitamin A within the body. LRAT′s plays a role in the visual cycle as it contributes to the regeneration of visual pigments in the retina. The expression of LRAT is regulated by dietary vitamin A levels, and it is found in various tissues, including the liver, lungs, testis, and the eye. LRAT is primarily localized to the endoplasmic reticulum. Mutations in the LRAT gene have been linked to a form of retinitis pigmentosa, an inherited retinal disease associated with progressive vision loss.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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