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AP189F

Sigma-Aldrich

Donkey Anti-Rat IgG Antibody, FITC conjugate, Species Adsorbed

1.5 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

FITC conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

concentration

1.5 mg/mL

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Antibody was isolated from whole goat antiserum by antigen affinity chromatography. Monospecific by IEP against whole rat serum. Reacts with heavy chain of Rat IgG, as well as the light chains from most rat immunoglobulin classes.

To ensure minimal cross-reactivity to non-target immunoglobulin, the antibody was solid-phase absorbed against serum proteins from bovine, chicken, goat, guinea pig, hamster (Syrian), horse, human, rabbit, and sheep.

F/P RATIO:

8.9 μg/mg; 3.3 moles FITC per mole IgG

Immunogen

Highly purified Rat IgG isolated from pooled normal Rat Serum.

Application

1:50 - 1:200 for most applications.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
This Donkey anti-Rat IgG Antibody, FITC conjugate, Species Adsorbed is validated for use in IF for the detection of Donkey Rat IgG.

Physical form

Freeze-dried powder.

Storage and Stability

Store at +2-8°C. Do not freeze. After opening, restore to 0.4mL with distilled water. As an undiluted liquid the product is stable at 2-8°C for several weeks. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. Working aliquots should be discarded after use. Expiration Date: 1 year from reconstitution.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Huang, YH; Ito, A; Arai, R
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Sara Baldassano et al.
American journal of physiology. Gastrointestinal and liver physiology, 302(3), G352-G358 (2011-11-15)
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