ABF124
Anti-cGAS Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CGAS(115004)
General description
Cyclic GMP-AMP synthase (cGAS), also known as cGAMP synthase, h-cGAS, Mab-21 domain-containing protein 1, and encoded by the gene name MB21D1 and C6orf150, is a member of the MAB-21 family. Cyclic GMP-AMP synthase (cGAS) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. The catalysis process involves both the formation of a 2′,5′ phosphodiester linkage at the GpA step and the formation of a 3′,5′ phosphodiester linkage at the ApG step, producing c[G(2′,5′)pA(3′,5′)p]. Recent studies have indicated that Cyclic GMP-AMP synthase (cGAS) has an antiviral activity by acting as a key cytosolic DNA sensor, when the presence of double-stranded DNA (dsDNA) in the cytoplasm acts as a danger signal that triggers the immune responses. Cyclic GMP-AMP synthase (cGAS) then binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. Cyclic GMP-AMP synthase (cGAS) is expressed in the monocytic cell line THP1.
Immunogen
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human cGAS near the C-terminus.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-cGAS antibody is validated for use in Western Blotting for the detection of cGAS.
Quality
Evaluated by Western Blotting in MDA-MB-231 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected cGAS in 10 µg of MDA-MB-231 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected cGAS in 10 µg of MDA-MB-231 cell lysate.
Target description
~54 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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