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582231

Sigma-Aldrich

Tetanus Toxoid

from Clostridium tetani, , lyophilized, nontoxic antigenic agent, Calbiochem®

Synonym(s):

TTx

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

Tetanus Toxoid, Clostridium tetani, This tetanus toxoid is prepared by formaldehyde inactivation of tetanus toxin. It retains its antigenic character, but is essentially nontoxic as determined by an LD₅₀ in mice.

Quality Level

200

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

General description

Tetanus Toxoid belongs to the vaccine class of medications. Useful for immunization against tetanus and wound prophylaxis. Non-toxic as determined by an LD50 assay in mice.

Application

Tetanus Toxoid, Clostridium tetani has been used: as a positive-control antigen used to assess antibodies against different SARS-CoV-2 proteins and peptides in patients; to assess lymphocyte proliferation in rabbit PBMC cultures in response to recall antigen stimulation; a stimulus for PBMCs from chronic HBV carrier donors in a T cell proliferation assay

Biochem/physiol Actions

Tetanus Toxoid acts against Clostridium tetani, a gram-positive bacillus that produces a neurotoxin called tetanospasmin. Tetanospasmin prevents the release of an inhibitory neurotransmitter and causes unopposed muscle contractions and spasms. Tetanus toxoid vaccine stimulates the immune system to respond to the vaccine’s antigens. The immune system’s T-lymphocytes (TH2 and B cells) secrete immunoglobulin against the antigen in the vaccine. This immunoglobulin helps to protect against future infections.

Features and Benefits

  • Prepared by formaldehyde inactivation of tetanus toxin.
  • Prepared by formaldehyde inactivation of tetanus toxin.
  • Toxoid retains its antigenic character, but is essentially nontoxic as determined by an LD50 in mice.
  • Cell permeable: no
  • Product does not compete with ATP.
  • Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM histidine, 100 mM NaCl, 5% trehalose, pH 7.0.

Reconstitution

Reconstitute in 50 µl of sterile distilled H₂O

Other Notes

Wrenger, S., et al. 2000. J. Biol. Chem.275, 22180.
Habermann E. and Goretzki, K. 1985. In Monoclonal Antibodies Against Bacteria (Macario, A.J.L., and Conway de Macario, E. eds.) Academic Press, New York, p.191.
Kozbor, D. and Roder, J.C. 1981. J. Immunol.127, 1275.
Bizzini, B., et al. 1970. Eur. J. Biochem.17, 100.
Largier, J.F. and Joubert, F.J. 1956. Biochem. Biophys. Acta20, 407.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Allan Ma et al.
Human vaccines & immunotherapeutics, 16(4), 756-778 (2019-11-07)
In chronic Hepatitis B Virus (HBV) infections HBV-specific T cells are functionally impaired. Immunotherapy may restore HBV-specific T cell responses essential for sustained disease remission off-treatment and induction of a functional cure. Chimigen® Molecules are fusion proteins of antigen(s) with

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