A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.

A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.

Biomedical chromatography : BMC (2016-08-25)

Khaled S Abdelkawy, Mark E Balyshev, Fawzy Elbarbry

ABSTRACT

A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC-18 T C

MATERIALS

Product Number

Brand

Product Description

59136-U

Supelco

SUPELCOSIL^™ LC-18-T HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

59564C30

Supelco

SUPELCOSIL^™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm

58928-U

Supelco

SUPELCOSIL^™ LC-18-S HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

59132

Supelco

SUPELCOSIL^™ LC-NH₂-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

58970-U

Supelco

SUPELCOSIL^™ LC-18-T HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

59621C30

Supelco

SUPELCOSIL^™ LC-18-T Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm

59613

Supelco

SUPELCOSIL^™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm

58970C30

Supelco

SUPELCOSIL^™ LC-18-T HPLC Column, 3 μm particle size, L × I.D. 15 cm × 3 mm

59621

Supelco

SUPELCOSIL^™ LC-18-T Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

59554

Supelco

SUPELCOSIL^™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

59620

Supelco

SUPELCOSIL^™ LC-18-T Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

59516

Supelco

SUPELCOSIL^™ LC-NH₂-NP Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

58931

Supelco

SUPELCOSIL^™ LC-18-S HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

59564

Supelco

SUPELCOSIL^™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

58971

Supelco

SUPELCOSIL^™ LC-18-T HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

57939

Supelco

SUPELCOSIL^™ LC-18-S HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm