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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Supelco
Tetrahydrofuran, HPLC grade, ≥99.9%, inhibitor-free
Sigma-Aldrich
Diethylene glycol, ReagentPlus®, 99%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
Acetone, ≥99%, meets FCC analytical specifications
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
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N,N-Dimethylformamide, anhydrous, 99.8%
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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Piperidine, ReagentPlus®, 99%
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N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Supelco
Acetone, analytical standard
Supelco
Diethyl ether, analytical standard
Supelco
Tetrahydrofuran, analytical standard
Supelco
Methanol, analytical standard
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
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Sodium chloride, tested according to Ph. Eur.
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Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
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Tetraethyl orthosilicate, ≥99.0% (GC)
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Diethylene glycol, BioUltra, ≥99.0% (GC)
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Supelco
(±)-Epichlorohydrin, analytical standard
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %
Sigma-Aldrich
Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
Sigma-Aldrich
Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
Supelco
Tetrahydrofuran, Selectophore, ≥99.5%
Sigma-Aldrich
Sodium chloride solution, 0.85%