- Preparation of peptide and other biomolecular conjugates through chemoselective ligations.
Preparation of peptide and other biomolecular conjugates through chemoselective ligations.
Methods in molecular biology (Clifton, N.J.) (2011-06-16)
Mathieu Galibert, Olivier Renaudet, Didier Boturyn, Pascal Dumy
PMID21674326
ABSTRACT
The synthesis of molecular conjugates through chemoselective ligations represents a very convenient strategy to prepare complex macromolecules with diverse functional elements. Herein, we describe chemical methods based on the preparation of chemoselectively addressable peptides allowing successive oxime ligations and/or alkyne-azide cycloaddition ("click") reactions of various biomolecules. This modular synthetic approach can be applied to a broad range of purposes.
MATERIALS