Skip to Content
Merck
All Photos(1)

Key Documents

SRP3252

Sigma-Aldrich

SDF-1 alpha from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

CXCL12, PBSF, Stromal-Cell Derived Factor-1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

50.0-100.0 ng/mL

mol wt

7.9 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

Q9QZD1

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... CXCL12(24772)

General description

Stromal cell-derived factor 1 α and β (SDF-1α and β) are stromal derived CXC chemokines, and signal through the C-X-C motif chemokine receptor-4 (CXCR4). SDF-1α is also known as C-X-C motif chemokine ligand 12 (CXCL12). Recombinant rat SDF-1α is a 7.9kDa protein containing 68 amino acid residues.

Application

Stromal cell-derived factor 1 α (SDF-1α ) has been used in cell migration assay and fibrin gel bead assay.

Biochem/physiol Actions

Stromal cell-derived factor 1 α and β (SDF-1α and β) chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. SDF-1 α modulates leukocyte and hematopoietic precursor migration and pre-B cell proliferation.

Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Stromal cell-derived factor 1a (SDF-1a): A marker of disease burden in patients with atrial fibrillation.
Li D
Scandinavian Cardiovascular Journal : SCJ, 50(1) , 35-41 (2016)
A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)
A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)
Bleul CC
The Journal of Experimental Medicine, 184(3), 1101-1109 (1996)
Expression of stress-response and cell proliferation genes in renal cell carcinoma induced by oxidative stress.
Tanaka T
The American Journal of Pathology, 156(6), 2149-2157 (2000)
MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation.
Papangeli I
Nature Communications, 7, 11268-11268 (2016)
Tanya Singh et al.
Journal of tissue engineering and regenerative medicine, 14(4), 609-621 (2020-02-23)
Neurodegeneration and dysfunction cause mobility impairment and/or paralysis in millions of adults, worldwide. Motor deficit and recovery in adults depend upon the plasticity of the neuromuscular junction (NMJ), a tripartite, biochemical synapse that transduces electrical impulses from the brain into
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service