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GC Analysis of FAMEs in Cookies on SP®-2560 after SPE Fractionation using Discovery® Ag-Ion

GC Analysis of FAMEs in Cookies on SP®-2560 after SPE Fractionation using Discovery® Ag-Ion suitable for GC, application for SPE

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09

SPE tube/cartridge

Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)

condition

4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant

sample addition

1 mL of extract; discard any eluant that drips through tube

elution

(Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum;(Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)

eluate post-treatment

evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

180 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen, 1 mL/min

injection

1 μL, 10:1 split

liner

4 mm I.D., split type, single taper wool packed FocusLiner design

Description

Analysis Note

A sample of commercially purchased cookies were prepared using an acid digestions /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (1). The resulting extracts were then fractionated using Ag-ion SPE and analyzed by GC/FID on the 100 m SP-2560 capillary column.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SP is a registered trademark of Sigma-Aldrich Co. LLC