G005711

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis^® Express RP Amide after Sample Prep using HybridSPE^®-Phospholipid

application for HPLC, application for SPE

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL; SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U); sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile; elution: apply vacuum; eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.; column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U); mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B); flow rate: 0.3 mL/min; column temp.: 35 °C; detector: ESI(+), m/z = 50-400; injection: 1 μL
• suitability: application for HPLC; application for SPE
• application(s): clinical

Description

Application

This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

Analysis Note

Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

5-Hydroxyomeprazole
Phenacetin
Omeprazole
Omeprazole sulfone