O4000

Oxythiamine chloride hydrochloride

≥95% (HPLC)

• Synonym(s): 5-(2-Hydroxyethyl)-3-(4-hydroxy-2-methyl-5-pyrimidinylmethyl)-4-methylthiazolium chloride
• Empirical Formula (Hill Notation): C₁₂H₁₆ClN₃O₂S · HCl
• CAS Number: 614-05-1
• Molecular Weight: 338.25
• MDL number: MFCD00050656
• UNSPSC Code: 12352116
• PubChem Substance ID: 24897990
• NACRES: NA.79

Properties

• Quality Level: 200
• Assay: ≥95% (HPLC)
• form: powder
• technique(s): HPLC: suitable
• color: white to off-white
• storage temp.: −20°C
• SMILES string: CC(N1)=NC=C(C[N+]2=CSC(CCO)=C2C)C1=O.Cl.[Cl-]
• InChI: 1S/C12H16N3O2S.2ClH/c1-8-11(3-4-16)18-7-15(8)6-10-5-13-9(2)14-12(10)17;;/h5,7,16H,3-4,6H2,1-2H3,(H,13,14,17);2*1H
• InChI key: QHUYPZVMEJLSEB-UHFFFAOYSA-N

Description

General description

Oxythiamine is a thiamine antimetabolite and the source through diet is by consuming acidic thiamine rich foods.

Application

Oxythiamine chloride hydrochloride is suitable for use:

• as a reference standard for calibration curve generation in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for oxythiamine pyrophosphate (OTPP) quantification in red blood cells
• as a thiamine transport inhibitor in human mammary epithelial cells (HMEC) and breast cancer cell lines
• in the synthesis of oxythiamineH picrolonate salt

Biochem/physiol Actions

Oxythiamine (OT) is a thiamine antagonist. It is a transketolase inhibitor and is employed to study anti-metastatic mechanisms, especially those involving metalloproteinases (MMP). It significantly sensitizes human hepatocellular carcinoma cells (HCC) to sorafenib, favoring tumor suppression.

Oxythiamine inhibits Transketolase, the enzyme that controls the nonoxidative branch of the pentose phosphate pathway.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves