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C5338

Sigma-Aldrich

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

lyophilized powder

Synonym(s):

Cyanogen bromide-activated Agarose

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About This Item

CAS Number:
68987-32-6
MDL number:
MFCD00163385
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4 Fast Flow

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 4-5 mL gel

particle size

45—165 μm

capacity

6-11 μmol/mL coupling capacity (6-AKS)

storage temp.

2-8°C

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General description

C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated-Sepharose® 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Application

Cyanogen bromide-activated-Sepharose™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Physical form

Lyophilized powder with sugar stabilizers

Legal Information

Sepabeads is a registered trademark of Mitsubishi Chemical Corp.
Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sepharose™ 6B 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)

Sigma-Aldrich

6B100

Sepharose™ 6B

Joyce M Bevington et al.
Virology, 357(1), 102-113 (2006-09-09)
Adeno-associated virus (AAV) is a human parvovirus that normally requires a helper virus such as adenovirus (Ad) for replication. The four replication proteins (Rep78, 68, 52 and 40) encoded by AAV are pleiotropic effectors of virus integration, replication, transcription and
F Jafri et al.
Biotechnology and bioengineering, 56(6), 605-609 (2008-07-22)
Polyclonal antibodies directed against the yeast invertase glycosyls were raised by immunizing rabbits with neoglycoprotein-I and neoglycoprotein-II. The neoglycoproteins were prepared by separately coupling the N-linked large and small molecular weight yeast invertase oligosaccharides respectively to bovine serum albumin with
M H Zeng et al.
Transplantation proceedings, 38(10), 3312-3314 (2006-12-19)
Human natural killer (NK) cells, which can mediate direct lysis of porcine endothelial cells, play an important role in xenograft rejection. HLA-G, which is a critical molecule in maintaining maternal immune tolerance of semi-allogenic fetus, is able to protect susceptible
Regina Maria Sousa de Araújo et al.
Plant science : an international journal of experimental plant biology, 183, 20-26 (2011-12-27)
Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. In this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. The lectin was thermo-stable, ion-independent and

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