HPLC Analysis of Nucleosides on Ascentis^® Si (HILIC Mode)

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 150 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 270 nm

injection

1 μL

sample

as indicated in mobile phase