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  • nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor.

nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor.

Scientific reports (2017-07-01)
N Facchinello, T Skobo, G Meneghetti, E Colletti, A Dinarello, N Tiso, R Costa, G Gioacchini, O Carnevali, F Argenton, L Colombo, L Dalla Valle
ABSTRACT

Glucocorticoids (GCs) play important roles in developmental and physiological processes through the transcriptional activity of their cognate receptor (Gr). Using CRISPR/Cas9 technology, we established a zebrafish null Gr mutant line and compared its phenotypes with wild type and a zebrafish line with partially silenced gr (gr

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
Dextran sulfate sodium salt, Mr ~40,000
Sigma-Aldrich
Triton X-100, laboratory grade