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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ABSTRACT

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 53.5-56.5% in H₂O

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Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

2-Bromo-2-methylpropionic acid, 98%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 1.0 M in methanol

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 40 wt. % in H₂O

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Copper(II) bromide, 99.999% trace metals basis

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Copper(II) bromide, 99%

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Acetone, histological grade, ≥99.5%

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Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

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Acetone, ACS reagent, ≥99.5%

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Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

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Acetone, ACS reagent, ≥99.5%

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Acetone, Laboratory Reagent, ≥99.5%

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L-Glutamine

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Deuterium oxide, filtered, 99.8 atom % D

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Deuterium oxide, 60 atom % D

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Deuterium oxide, 70 atom % D

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Bis(4-nitrophenyl) carbonate, ≥99%

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, ≥99.0% (AT)

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Ethylenediamine solution, technical, 75-80%

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L-Glutamine, BioUltra, ≥99.5% (NT)

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Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)

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Diethylamine, puriss. p.a., ≥99.5% (GC)

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Deuterium oxide, 99.9 atom % D, contains 1 % (w/w) 3-(trimethylsilyl)-1-propanesulfonic acid, sodium salt (DSS)

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Diethylamine, purified by redistillation, 99.5%

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Ethylenediamine, ReagentPlus^®, ≥99%

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Deuterium oxide, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

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1,2-Dichloroethane, anhydrous, 99.8%