- Determination of polyamines in hydrolysates of uremic plasma by high-performance cation-exchange column chromatography.
Determination of polyamines in hydrolysates of uremic plasma by high-performance cation-exchange column chromatography.
Journal of chromatography (1983-02-11)
T Takagi, T G Chung, A Saito
PMID6833425
ABSTRACT
The levels of putrescine, cadaverine, spermidine and spermine in uremic plasma were determined with an automatic polyamine analyzer with a 7.5 X 0.2 cm I.D. cation-exchange column using a stepwise sodium chloride gradient. All four polyamines were higher in ten patients with chronic renal failure than in eight normal subjects. The total polyamine content was also measured in the patients' plasma before and after maintenance dialysis; putrescine and spermidine levels were significantly lowered by the procedure.
MATERIALS
Product Number
Brand
Product Description
Supelco
Ethanol-20 (10 ampules/kit), 20 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-500, 500 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Supelco
Ethanol-400 (10 ampules/kit), 400 mg/dL in H2O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®
Sigma-Aldrich
Trimethylamine solution, 31-35 wt. % in ethanol, 4.2 M, contains toluene as stabilizer
Supelco
Ethanol Calibration Kit, ampule of 10 × 1.2 mL, certified reference material, Cerilliant®