LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.

LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.

Antimicrobial agents and chemotherapy (2010-09-22)

Jody L Floyd, Kenneth P Smith, Sanath H Kumar, Jared T Floyd, Manuel F Varela

PMID20855745

ABSTRACT

A multidrug efflux pump designated LmrS (lincomycin resistance protein of Staphylococcus aureus), belonging to the major facilitator superfamily (MFS) of transporters, was cloned, and the role of LmrS in antimicrobial efflux was evaluated. The highest relative increase in MIC, 16-fold, was observed for linezolid and tetraphenylphosphonium chloride (TPCL), followed by an 8-fold increase for sodium dodecyl sulfate (SDS), trimethoprim, and chloramphenicol. LmrS has 14 predicted membrane-spanning domains and is homologous to putative lincomycin resistance proteins of Bacillus spp., Lactobacillus spp., and Listeria spp.

MATERIALS

Product Number

Brand

Product Description

46794

Supelco

Sulfadimethoxine, VETRANAL^®, analytical standard

55422

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

71726

Supelco

Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

46984

Supelco

Trimethoprim, VETRANAL^®, analytical standard

92131

Sigma-Aldrich

Trimethoprim, ≥99.0% (HPLC)

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

218790

Sigma-Aldrich

Tetraphenylphosphonium chloride, 98%

45674

Sigma-Aldrich

Erythromycin, tested according to Ph. Eur.

R7382

Sigma-Aldrich

Rifampicin, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals

E6376

Sigma-Aldrich

Erythromycin, potency: ≥850 μg per mg

E5389

Sigma-Aldrich

Erythromycin, BioReagent, suitable for cell culture

S7007

Supelco

Sulfadimethoxine, 98.0-102.0%

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

E1385

Sigma-Aldrich

Ethidium bromide solution, BioReagent, for molecular biology, 500 μg/mL in H₂O

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

T7883

Sigma-Aldrich

Trimethoprim, ≥98.5%

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

R3501

Sigma-Aldrich

Rifampicin, ≥95% (HPLC), powder or crystals

E0774

Sigma-Aldrich

Erythromycin, meets USP testing specifications

46067

Sigma-Aldrich

Ethidium bromide solution, for fluorescence, ~1% in H₂O

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

RDD040

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

43530

Supelco

Reserpine Standard for LC-MS, analytical standard, for LC-MS

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

52365

Sigma-Aldrich

Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)

52370

Sigma-Aldrich

Hexadecyltrimethylammonium bromide, ≥96.0% (AT)