G005882

LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis^® Express C18

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U); mobile phase: [A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 0.1% formic acid acetonitrile; gradient: 10 to 70% B in 5 min; held at 70% B for 3 min; flow rate: 0.6 mL/min; pressure: 3698 psi (255 bar); column temp.: 35 °C; detector: MS, ESI(+), TIC, m/z 100-1000; injection: 5 μL; sample: 1000 ng/mL in 40:60 (water:methanol)
• suitability: application for HPLC
• application(s): clinical

Description

Analysis Note

An Ascentis Expres F5 column provided rapid, baseline resolution of testosterone and metabolites. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
17α-Methyltestosterone solution, 1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant^®
5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
6β-Hydroxytestosterone solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®