ZRB2478

Anti-DPP9 Antibody, clone 1E12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): DP9, DPLP9, DPP IX, DPRP-2, Dipeptidyl peptidase 9, Dipeptidyl peptidase IV-related protein 2, Dipeptidyl peptidase IX, Dipeptidyl peptidase-like protein 9
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1E12 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 98.26 kDa; observed mol wt ~98 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: (Blast lists NP_001371552 but uniprot shows NP_631898)
• UniProt accession no.: Q86TI2
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... DPP9(91039)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1E12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Dipeptidyl peptidase 9 (DPP9). It targets an epitope within 21 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Dipeptidyl peptidase 9 (DPP9).

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected DPP9 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected DPP9 in lysates from Jurkat and NTERA-2 cells and Human liver tissue..

Affinity Binding Assay: A representative lot of this antibody bound DPP9 peptide with a KD of 6.0 x 10-9 in an affinity binding assay.

Flow Cytometry Analysis: 1 µg from a representative lot detected DPP9 in one million NTERA-2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DPP9 in Human liver tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DPP9 in HT-29 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Dipeptidyl peptidase 9 (UniProt: Q86TI2; also known as EC:3.4.14.5, DP9, Dipeptidyl peptidase IV-related protein 2, DPRP-2, Dipeptidyl peptidase IX, DPP IX, Dipeptidyl peptidase-like protein 9, DPLP9) is encoded by the DPP9 (also known as DPRP2) gene (Gene ID: 9039) in human. DPP9 is a ubiquitously expressed enzyme that cleaves off N-terminal dipeptides from proteins having a proline or alanine a residue at position 2. Its highest levels are observed in liver, heart, and muscle and low levels are detected in brain. Three isoforms of DPP9 have been described. Isoform 1 is cytosolic and isoform 2 is detected in the nucleus and contains a nuclear localization signal (aa 2-9). A third isoform has also been described that lacks amino acids 832-858. Although DPP9 is associated with mitochondria, the strongest co-localization is observed with microtubules. It participates in a number of cellular processes, including extracellular matrix interactions, cell proliferation, and apoptosis. Knockdown of DPP9 can induce neuropeptide Y-driven tumor cell death, mediated by poly (ADP-ribose) polymerase-1 (PARP-1) and apoptosis-inducing factor (AIF). DPP9 also acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing the activation of NLRP1 and CARD8. Silencing of DPP9 is reported to reduce Cbl interaction with Syk. It preferentially cleaves the active form of Syk and acts as a negative regulator to shut-off Syk after B cell stimulation, suggesting that DPP9 can influence how much and how long the B cells respond to stimulation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Justa-Schuch, D., et al. (2016). eLife 5: e16370; Zhang, H., et al. (2015). Biochim. Biophys. Acta. 1853(2); 470-480; Lu, C., et al. (2011). J. Biol. Chem. 286(31); 27494-27505)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable