SRP0339

Ceramide Kinase FLAG tag active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)

• Synonym(s): CERK, LK4, hCERK
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥40% (SDS-PAGE)
• form: aqueous solution
• mol wt: 60 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 0.4 mg/mL
• NCBI accession no.: NM_022766
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CERK(64781)

Description

General description

Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain.[1] The gene encoding CERK is localized on human chromosome 22q13.31.[2]

Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate.[3][4] It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling.[5] The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.[6]

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable