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Key Documents

SAB4503810

Sigma-Aldrich

Anti-phospho-CDC25B (pSer323) antibody produced in rabbit

affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 64 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
western blot: 1:500-1:1000

NCBI accession no.

P30305

UniProt accession no.

P30305

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer323)

Gene Information

human ... CDC25B(994)

General description

CDC25B (cell division cycle 25B), a short half-life protein belongs to the dual-specificity CDC25 phosphatases family. It is located on human chromosome 20p. The protein is upregulated in late G2 of the cell cycle.

Immunogen

The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323.

Immunogen Range: 289-338

Biochem/physiol Actions

The expression of CDC25B (cell division cycle 25B) can be considered as a prognostic marker in the pathogenesis of retinoblastoma. It helps to dephosphorylate CDK1 (cyclin-dependent kinase 1) and ERK1/2 (extracellular signal-regulated kinase 1/2). It controls the cell cycle.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression of CDC25A and CDC25B phosphatase proteins in human retinoblastoma and its correlation with clinicopathological parameters
Singh L, et al.
The British Journal of Ophthalmology, 99(4), 457-463 (2015)
Targeting cell division cycle 25 homolog B to regulate influenza virus replication
Perwitasari O, et al.
Journal of Virology, 87(24), 13775-13784 (2013)
Comparative genomic hybridization analysis of genetic aberrations associated with development of esophageal squamous cell carcinoma in Henan, China
Qin YR, et al.
World Journal of Gastroenterology, 14(12), 1828-1835 (2008)
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition
Dutertre S, et al.
Journal of Cell Science (2004)

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