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  • Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products.

Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products.

Natural product communications (2011-05-28)
Yuan-Chun Ma, Xiao-Qiang Wang, Fei Fei Hou, Jie Ma, Mai Luo, Shane Lu, Peter Jin, Nelly Terevsky, Alice Chen, Iris Xu, Asmita V Patel, Dariusz Gorecki
ABSTRACT

A simple, sensitive and reliable reversed phase Rapid Resolution Liquid Chromatography (RRLC) method was developed and validated for six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin and rosiridin) in Rhodiola rosea L. roots and powder extracts. The method uses a Phenomenex C18 (2)-HST column at 40 degrees C with a neutral gradient system mobile phase (H20 and acetonitrile), a flow rate of 1.0 mL/min, and UV detection wavelengths set at 205 and 254 nm, simultaneously. Baseline separation of the six active compounds was achieved within 8 minutes. The average percentages of rosavins (rosarin, rosavin, and rosin) in authentic R. rosea roots and root powder extracts were quantitatively determined and a characteristic R. rosea roots RRLC profile was established. The RRLC method is accurate and sensitive; in addition, it effectively increases the sample analysis throughput compared with conventional HPLC.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gum rosin, CP
Sigma-Aldrich
Gum rosin, natural resin