504971-40

Discovery^® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

• Synonym(s): C18 TM=["Discovery"] HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Discovery^® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
• material: stainless steel column
• Agency: suitable for USP L1
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4 mm
• surface area: 300 m²/g
• surface coverage: 3 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 180 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Application

• Identification, isolation and characterization of dolutegravir forced degradation products and their cytotoxicity potential.: Utilizes the Discovery^® C18 HPLC Column to study the stability and degradation of the antiretroviral drug dolutegravir, crucial for ensuring drug safety and efficacy (Saida et al., 2019).


• Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid beta 1-42.: This methodological study leverages the Discovery^® C18 HPLC Column for the purification and analysis of amyloid beta peptides, contributing to research on Alzheimer′s disease (Prakash et al., 2019).


• Development and validation of a HPLC electrochemical detection method to measure COMT activity as a tool in drug development.: Showcases the use of the Discovery^® C18 HPLC Column in developing a novel analytical method for measuring catechol-O-methyltransferase (COMT) activity, instrumental in the development of psychiatric and neurological medications (Viljoen et al., 2019).

Features and Benefits

• Excellent reproducibility
• Exceptional peak shape for basic and acidic analytes
• Stable, low-bleed LC-MS separations
• Separation of peptides and small proteins
• Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable