75315

OF Test Nutrient Agar

suitable for microbiology, NutriSelect^® Basic

• Synonym(s): Oxidative Fermentative Agar acc. to Hugh a. Leifson
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 2.5 g/L ; bromothymol blue, 0.08 g/L ; casein peptone (pancreatic), 2 g/L ; dipotassium hydrogen phosphate, 0.3 g/L ; sodium chloride, 5 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | utilization test: suitable; microbiological culture: suitable
• final pH: 7.1±0.2 (25 °C)
• antibiotic activity spectrum: Gram-negative bacteria
• application(s): agriculture; clinical testing; environmental; food and beverages; microbiology
• suitability: Alcaligenes spp.; Chromobacterium spp.; Moraxella spp.; Pasteurella spp.; Acinetobacter spp.; Aeromonas spp.; flavobacteria; nonselective and differential for Bordetella spp.; nonselective and differential for Campylobacter spp.; nonselective and differential for Enterococcus spp.; nonselective and differential for Escherichia coli; nonselective and differential for Pseudomonas spp.; nonselective and differential for Salmonella spp.; nonselective and differential for Shigella spp.; nonselective and differential for Vibrio spp.; nonselective and differential for Yersinia spp.; nonselective and differential for bacteria (General Media); nonselective and differential for coliforms; nonselective and differential for enterobacteriaceae

Description

Application

Medium for demonstrating oxidative and fermentative catabolism of carbohydrate acc. to Hugh and Leifson (1953). In particular for the differentiation and classification of gram-negative intestinal bacteria.

Preparation Note

Dissolve 9.88 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Let cool to approx. 50°C and add 100 ml of sterile filtered 10% D(+)-glucose (Cat. No. 49140), lactose (Cat. No. 61340), sucrose (Cat. No. 84100) or other carbohydrate.

Other Notes

Studies on coagulase-positive staphylococci from bovine milk

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves